Molecular Characterization of a Pathogen-Inducible Bidirectional Promoter from Hot Pepper (Capsicum annuum).
Identifieur interne : 000120 ( Main/Exploration ); précédent : 000119; suivant : 000121Molecular Characterization of a Pathogen-Inducible Bidirectional Promoter from Hot Pepper (Capsicum annuum).
Auteurs : Solhee In [Corée du Sud] ; Hyun-Ah Lee [Corée du Sud] ; Jongchan Woo [Corée du Sud, États-Unis] ; Eunsook Park [Corée du Sud, États-Unis] ; Doil Choi [Corée du Sud]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Capsicum, Maladies des plantes, Protéines végétales.
- microbiologie : Maladies des plantes.
- pathogénicité : Phytophthora, Pseudomonas syringae.
- Régions promotrices (génétique), Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Capsicum, Plant Diseases.
- microbiology : Plant Diseases.
- pathogenicity : Phytophthora, Pseudomonas syringae.
- Gene Expression Regulation, Plant, Promoter Regions, Genetic.
Abstract
In hot pepper, the sesquiterpene phytoalexin capsidiol is catalyzed by the two final-step enzymes, a sesquiterpene cyclase (EAS) and a hydroxylase (EAH), which are genetically linked and present as head-to-head orientation in the genome. Transcriptomic analysis revealed that a subset of EAS and EAH is highly induced following pathogen infection, suggesting the coregulation of EAS and EAH by a potential bidirectional activity of the promoter (pCaD). A series of the nested deletions of pCaD in both directions verified the bidirectional promoter activity of the pCaD. Promoter deletion analysis revealed that the 226 bp of the adjacent promoter region of EAS and GCC-box in EAH orientation were determined as critical regulatory elements for the induction of each gene. Based on promoter analyses, we generated a set of synthetic promoters to maximize reporter gene expression within the minimal length of the promoter in both directions. We found that the reporter gene expression was remarkably induced upon infection with Phytophthora capsici, Phytophthora infestans, and bacterial pathogen Pseudomonas syringae pv. tomato DC3000 but not with necrotrophic fungi Botrytis cinerea. Our results confirmed the bidirectional activity of the pCaD located between the head-to-head oriented phytoalexin biosynthetic genes in hot pepper. Furthermore, the synthetic promoter modified in pCaD could be a potential tool for pathogen-inducible expression of target genes for developing disease-resistant crops.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
DOI: 10.1094/MPMI-07-20-0183-R
PubMed: 32781924
Affiliations:
Links toward previous steps (curation, corpus...)
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xml:lang="en"><term>Capsicum (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Pseudomonas syringae (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Capsicum (génétique)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (pathogénicité)</term><term>Protéines végétales (génétique)</term><term>Pseudomonas syringae (pathogénicité)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Capsicum</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Capsicum</term><term>Maladies des plantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In hot pepper, the sesquiterpene phytoalexin capsidiol is catalyzed by the two final-step enzymes, a sesquiterpene cyclase (EAS) and a hydroxylase (EAH), which are genetically linked and present as head-to-head orientation in the genome. Transcriptomic analysis revealed that a subset of <i>EAS</i> and <i>EAH</i> is highly induced following pathogen infection, suggesting the coregulation of <i>EAS</i> and <i>EAH</i> by a potential bidirectional activity of the promoter (pCaD). A series of the nested deletions of pCaD in both directions verified the bidirectional promoter activity of the pCaD. Promoter deletion analysis revealed that the 226 bp of the adjacent promoter region of <i>EAS</i> and GCC-box in <i>EAH</i> orientation were determined as critical regulatory elements for the induction of each gene. Based on promoter analyses, we generated a set of synthetic promoters to maximize reporter gene expression within the minimal length of the promoter in both directions. We found that the reporter gene expression was remarkably induced upon infection with <i>Phytophthora capsici</i>, <i>Phytophthora infestans</i>, and bacterial pathogen <i>Pseudomonas syringae</i> pv. tomato DC3000 but not with necrotrophic fungi <i>Botrytis cinerea</i>. Our results confirmed the bidirectional activity of the pCaD located between the head-to-head oriented phytoalexin biosynthetic genes in hot pepper. Furthermore, the synthetic promoter modified in pCaD could be a potential tool for pathogen-inducible expression of target genes for developing disease-resistant crops.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">32781924</PMID><DateCompleted><Year>2020</Year><Month>11</Month><Day>16</Day></DateCompleted><DateRevised><Year>2020</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Molecular Characterization of a Pathogen-Inducible Bidirectional Promoter from Hot Pepper (<i>Capsicum annuum</i>).</ArticleTitle><Pagination><MedlinePgn>1330-1339</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-07-20-0183-R</ELocationID><Abstract><AbstractText>In hot pepper, the sesquiterpene phytoalexin capsidiol is catalyzed by the two final-step enzymes, a sesquiterpene cyclase (EAS) and a hydroxylase (EAH), which are genetically linked and present as head-to-head orientation in the genome. Transcriptomic analysis revealed that a subset of <i>EAS</i> and <i>EAH</i> is highly induced following pathogen infection, suggesting the coregulation of <i>EAS</i> and <i>EAH</i> by a potential bidirectional activity of the promoter (pCaD). A series of the nested deletions of pCaD in both directions verified the bidirectional promoter activity of the pCaD. Promoter deletion analysis revealed that the 226 bp of the adjacent promoter region of <i>EAS</i> and GCC-box in <i>EAH</i> orientation were determined as critical regulatory elements for the induction of each gene. Based on promoter analyses, we generated a set of synthetic promoters to maximize reporter gene expression within the minimal length of the promoter in both directions. We found that the reporter gene expression was remarkably induced upon infection with <i>Phytophthora capsici</i>, <i>Phytophthora infestans</i>, and bacterial pathogen <i>Pseudomonas syringae</i> pv. tomato DC3000 but not with necrotrophic fungi <i>Botrytis cinerea</i>. Our results confirmed the bidirectional activity of the pCaD located between the head-to-head oriented phytoalexin biosynthetic genes in hot pepper. Furthermore, the synthetic promoter modified in pCaD could be a potential tool for pathogen-inducible expression of target genes for developing disease-resistant crops.[Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>In</LastName><ForeName>Solhee</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Immunity Research Center, Seoul National University, Seoul 08826, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyun-Ah</ForeName><Initials>HA</Initials><AffiliationInfo><Affiliation>Division of Eco-Friendly Horticulture, Yonam College, Cheonan 31005, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Woo</LastName><ForeName>Jongchan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Plant Immunity Research Center, Seoul National University, Seoul 08826, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Eunsook</ForeName><Initials>E</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-2984-3039</Identifier><AffiliationInfo><Affiliation>Plant Immunity Research Center, Seoul National University, Seoul 08826, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, U.S.A.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Doil</ForeName><Initials>D</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-4366-3627</Identifier><AffiliationInfo><Affiliation>Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Immunity Research Center, Seoul National University, Seoul 08826, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002212" MajorTopicYN="Y">Capsicum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044224" MajorTopicYN="N">Pseudomonas syringae</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">disease resistance</Keyword><Keyword MajorTopicYN="N">hot pepper</Keyword><Keyword MajorTopicYN="N">pathogen-inducible promoter</Keyword><Keyword MajorTopicYN="N">phytoalexin</Keyword><Keyword MajorTopicYN="N">transient expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32781924</ArticleId><ArticleId IdType="doi">10.1094/MPMI-07-20-0183-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>États-Unis</li></country><region><li>Région capitale de Séoul</li><li>Wyoming</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="In, Solhee" sort="In, Solhee" uniqKey="In S" first="Solhee" last="In">Solhee In</name></region><name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name><name sortKey="Choi, Doil" sort="Choi, Doil" uniqKey="Choi D" first="Doil" last="Choi">Doil Choi</name><name sortKey="In, Solhee" sort="In, Solhee" uniqKey="In S" first="Solhee" last="In">Solhee In</name><name sortKey="Lee, Hyun Ah" sort="Lee, Hyun Ah" uniqKey="Lee H" first="Hyun-Ah" last="Lee">Hyun-Ah Lee</name><name sortKey="Park, Eunsook" sort="Park, Eunsook" uniqKey="Park E" first="Eunsook" last="Park">Eunsook Park</name><name sortKey="Woo, Jongchan" sort="Woo, Jongchan" uniqKey="Woo J" first="Jongchan" last="Woo">Jongchan Woo</name></country><country name="États-Unis"><region name="Wyoming"><name sortKey="Woo, Jongchan" sort="Woo, Jongchan" uniqKey="Woo J" first="Jongchan" last="Woo">Jongchan Woo</name></region><name sortKey="Park, Eunsook" sort="Park, Eunsook" uniqKey="Park E" first="Eunsook" last="Park">Eunsook Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Molecular Characterization of a Pathogen-Inducible Bidirectional Promoter from Hot Pepper (Capsicum annuum).
}}
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